Selected Publications

• Manik C. Ghosh, Wing-Hang Tong, Deliang Zhang, Hayden Ollivierre-Wilson, Anamika Singh, Murali C. Krishna, James B. Mitchell, and Tracey A. Rouault
  (2008) Tempol-mediated activation of latent iron regulatory protein activity prevents symptoms of neurodegenerative disease in IRP2 knockout mice
  PNAS. 2008 August 19;105:12028-12033 PDF
• Tracey A. Rouault and Wing Hang Tong
  (2008) Iron-sulfur cluster biogenesis and human disease.
  Trends in Genetics. 5 July 2008. [Epub ahead of print] PDF
• Li K, Besse EK, Ha D, Kovtunovych G, Rouault TA.
  (2008) Iron-dependent regulation of frataxin expression: implications for treatment of Friedreich ataxia.
  Hum Mol Genet. 2008 Apr 17. [Epub ahead of print] PDF
• Mochel F, Knight MA, Tong WH, Hernandez D, Ayyad K, Taivassalo T, Andersen PM, Singleton A, Rouault TA, Fischbeck KH, Haller RG.
  (2008) Splice mutation in the iron-sulfur cluster scaffold protein ISCU causes myopathy with exercise intolerance.
  Am J Hum Genet. 2008 Mar;82(3):652-60. Epub Feb 14. PDF
• Missirlis F, Kosmidis S, Brody T, Mavrakis M, Holmberg S, Odenwald WF, Skoulakis EM, Rouault TA.
  (2007) Homeostatic mechanisms for iron storage revealed by genetic manipulations and live imaging of Drosophila ferritin. Genetics. 2007 Sep;177(1):89-100. Epub 2007 Jul 1. PDF
• Zhang, D., Meyron-Holtz, E. and Rouault, T. A.
  (2007) Renal Iron Metabolism: Transferrin Iron Delivery and the Role of Iron Regulatory Proteins. J Am Soc Nephrol 18:401-406. PDF
• Tong, W. H. and Rouault, T. A.
  (2007) Metabolic regulation of citrate and iron by aconitases: role of iron–sulfur cluster biogenesis. Biometals. In press. PDF
• Rouault, T. A.
  (2006) If the RNA fits, use it. Science. 314:1886-87. PDF
• Rouault, T. A. and Cooperman, S.
  (2006) Brain Iron Metabolism. Semin Pediatr Neurol. 13:142-148. PDF.
  
• Rouault, T. A.
  (2006)The role of iron regulatory proteins in mammalian iron homeostasis and disease. Nat. Chem. Biol. 2:406-414. PDF.
• Smith S. R., Ghosh M. C., Ollivierre-Wilson, H., Tong, W. H., Rouault T. A.
  (2006) Complete loss of iron regulatory proteins 1 and 2 prevents viability of murine zygotes beyond the blastocyst stage of embryonic development. Blood Cells Mol Dis. In press. PDF
• Li K., Tong W. H., Hughes R. M., Rouault T. A.
  (2006) Roles of the mammalian cytosolic cysteine desulfurase, ISCS, and scaffold protein, ISCU in iron-sulfur cluster assembly. J Biol Chem. In press. PDF
  
• Tong W. H., Rouault, T. A.
  (2006) Functions of mitochondrial ISCU and cytosolic ISCU in mammalian iron-sulfur cluster biogenesis and iron homeostasis. Cell Metab. 3:199-210. PDF
• Wang, R. H., Li, C. Xu X., Zheng Y., Xiao, C., Zerfas, P., Cooperman, S., Eckhaus, M., Rouault, T. A., Mishra, L., Deng C. X.
  (2005) A role of SMAD4 in iron metabolism through the positive regulation of hepcidin expression. Cell Metab. 2:399-409. PDF
• Rouault, T. A.
  (2005) The intestinal heme transporter revealed. Cell 122:649-651. PDF
• Rouault, T. A.
  (2005) Linking Physiological functions of iron. Nat. Chem. Biol. 4: 193-194. PDF
• Cooperman, S. S., Meyron-Holtz, E. G., Olivierre-Wilson, H., Ghosh, M. C., McConnell, J. P., Rouault, T. A.
  (2005) Microcytic anemia, erythropoietic protoporphyria, and neurodegeneration in mice targeted deletion of iron-regulatory protein 2. Blood 106: 1084-1091. PDF
  
• Yikilmaz E., Rouault T. A., Schuck, P.
  (2005) Self-Associated and Ligand-Induced Conformational Changes of Iron Regulatory Proteins 1 and 2. Biochemistry 44:870-8478. PDF
  
• Zhang P., Land W., Lee S., Juliani J., Lefman J., Smith S. R., Germain D., Kessel M., Leapman R., Rouault T. A., Subramaniam S.
  (2005) Abstract Electron tomography of degenerating neurons in mice with abnormal regulation of iron metabolism. J Struct Biol. 150:144-53. PDF
  
• Rouault, T. A., Tong W.H.
  (2005) Opinion: Iron-sulphur cluster biogenesis and mitochondrial iron homeostasis.Nat Rev Mol Cell Biol. 6:345-51. PDF
  
• Meyron-Holtz, E. G., Ghosh, M. C., Rouault, T. A.
  (2004) Mammalian Tissue Oxygen Levels Modulate Iron Regulatory Protein Activities in Vivo. Science 306:2087-2090. PDF
  
• Rouault, T. A.
  (2004) Pathogenic Bacteria Prefer Heme. Science 305:1577-1578. PDF
  
• Smith, S. R., Cooperman, S., Lavaute, T., Tresser, N., Ghosh, M. Meyron-Holtz, E., Land, W., Ollivierre, H., Jortner, B., Switzer, R., Messing, A., Rouault, T. A.
  (2004) Severity of Neurodegeneration correlates with compromise of iron metabolism in mice with iron regulatory protein deficiencies. Ann. N.Y. Acad. Sci. 1012:65-83. PDF
  
• Wu, L. J., Leenders, A.G.M., Cooperman, S., Meyron-Holtz, E., Smith, S., Land, W., Tsai, R.Y.L., Berger, U.V., Sheng, Z.H., Rouault, T.A.
  (2004) Expression of the iron transporter ferroportin in synaptic vesicles and the blood-brain barrier. Brain Res., 1001:108-117. PDF
  
• Meyron-Holtz, E.G., Ghosh, M.C., Iwai, K., LaVaute, T., Brazzolotto, X., Berger, U. V., Land, W., Ollivierre-Willson, H., Grinberg, A., Love, P., Rouault, T.A.
  (2004) Genetic ablations of iron regulatory proteins 1 and 2 reveal why iron regulatory protein 2 dominates iron homeostasis. EMBO J, 23:386-395. PDF
  
• Allerson, C. R., Martinez, A., Yikilmaz, E., Rouault, T. A.
  (2003) A high-capacity RNA affinity column for the purification of human IRP1 and IRP2 overexpressed in Pichia pastoris. RNA, 9:364-374. PDF
  
• Bourdon, E., Kang, D. K., Ghosh, M. C., Drake, S.K., Wey, J., Levine, R. L., and Rouault, T. A.
  (2003). The role of endogenous heme synthesis and degradation domain cysteines in cellular iron-dependent degradation of IRP2. Blood Cells Mol Dis., 31, 247-55. PDF
  
• Tong, W. H., Jameson, G. N. L., Huynh, B. H., and Rouault, T. A.
  (2003). Subcellular compartmentalization of human Nfu, an iron–sulfur cluster scaffold protein, and its ability to assemble a [4Fe– 4S] cluster. Proc Natl Acad Sci U S A. 100, 9762-7. PDF
  
• Grabill, A., Silva A. C., Smith, S. S., Koretsky, A.P., Rouault, T. A.
  (2003). MRI detection of ferritin iron overload and associated neuronal pathology in iron regulatory protein-2 knockout mice. Brain Res., 971, 95-106. PDF
  
• Drake, S. K., Bourdon, E., Wehr, N. B., Levine, R. L., Backlund, P. S., Yergey, A. L., Rouault, T. A.
  (2002). Numerous Proteins in Mammalian Cells Are Prone to Iron-Dependent Oxidation and Proteasomal Degradation. Dev Neurosci., 24, 114-24. PDF
  
• Rouault T.A.
  (2001). Iron on the brain, Nature Genetics 28, 299-300. PDF
  
• LaVaute, T., Smith, S., Cooperman, S., Iwai, K., Land, W., Meyron-Holtz, E., Drake, S.K. Miller, G., Abu-Asab, M., Tsokos, M., Switzer III, R., Grinberg, A , Love, P. , Tresser, N., Rouault, T.A.
  (2001). Targeted deletion of iron regulatory protein 2 causes misregulation of iron metabolism and neurodegenerative disease in mice. Nature Genetics 27, 209-214. PDF
  
• Tong, W.H. and Rouault, T.A.
  (2000). Distinct iron-sulfur cluster assembly complexes exist in the cytosol and mitochondria of human cells. EMBO J 21, 5692-5700. PDF.
  
• Allerson, C. R., Cazzola, M., and Rouault, T. A.
  (1999). Clinical severity and thermodynamic effects of iron-responsive element mutations in hereditary hyperferritinemia-cataract syndrome. J Biol Chem 274, 26439-26447. PDF
  
• Land, T., and Rouault, T. A.
  (1998). Targeting of a human iron-sulfur cluster assembly enzyme, nifs, to different subcellular compartments is regulated through alternative AUG utilization. Mol Cell 2, 807-815. PDF
  
• Iwai, K., Drake, S. K., Wehr, N. B., Weissman, A. M., LaVaute, T., Minato, N., Klausner, R. D., Levine, R. L., and Rouault, T. A.
  (1998). Iron-dependent oxidation, ubiquitination, and degradation of iron regulatory protein 2: implications for degradation of oxidized proteins. Proc Natl Acad Sci U S A 95, 4924-4928. PDF
  
• McNamara, L., MacPhail, A. P., Gordeuk, V. R., Hasstedt, S. J., and Rouault, T.
  (1998). Is there a link between African iron overload and the described mutations of the hereditary haemochromatosis gene? Br J Haematol 102, 1176-1178.
  
• Addess, K. J., Basilion, J. P., Klausner, R. D., Rouault, T. A., and Pardi, A. J.
  (1997). Structure and Dynamics of the Iron Responsive Element RNA: Implications for Binding of the RNA by Iron Regulatory Proteins. J. Mol. Biol. 274, 72-83. PDF
  
• Rouault, T., and Klausner, R.
  (1997). Regulation of iron metabolism in eukaryotes. Curr Top Cell Regul 35, 1-19.
  
• Butt, J., Kim, H. Y., Basilion, J. P., Cohen, S., Iwai, K., Philpott, C. C., Altschul, S., Klausner, R. D., and Rouault, T. A.
  (1996). Differences in the RNA binding sites of iron regulatory proteins and potential target diversity. Proc. Natl. Acad. Sci.USA 93, 4345-4349. PDF
  
• Kim, H. Y., LaVaute, T., Iwai, K., Klausner, R. D., and Rouault, T. A.
  (1996). Identification of a conserved and functional iron-responsive element in the 5'UTR of mammalian mitochondrial aconitase. J Biol Chem 271, 24226-24230. PDF
  
• Rouault, T. A., and Klausner, R. D.
  (1996). Iron-sulfur clusters as biosensors of oxidants and iron. Trends in Biochem. Sci. 21, 174-177. PDF
  
• Rouault, T. A., Klausner, R. D., and Harford, J. B.
  (1996). Translational Control of Ferritin. Cold Spring Harbor Press), pp. 335-362.
  
• Rouault,T.A., and Klausner, R.D.
  (1996). Post-transcriptional regulation of genes of iron metabolism in mammalian cells. J Bioinorganic Chem 1, 494-498.
  
• Iwai, K., Klausner, R. D., and Rouault, T. A.
  (1995). Requirements for iron-regulated degradation of the RNA binding protein, iron regulatory protein 2. EMBO J. 14, 5350-5357.
  
• Kim, H. Y., Klausner, R. D., and Rouault, T. A.
  (1995). Translational repressor activity is equivalent and is quantitatively predicted by in vitro RNA binding for two iron-responsive element binding proteins, IRP1 and IRP2. J. Biol. Chem. 270, 4983-4986.
  
• Basilion, J. P., Rouault, T. A., Massinople, C. M., Klausner, R. D., and Burgess, W. H.
  (1994). The iron-responsive element-binding protein: Localization of the RNA binding site to the aconitase active-site cleft. Proc. Natl. Acad. Sci. U.S.A. 91, 574-578.
  
• Philpott, C. C., Klausner, R. D., and Rouault, T. A.
  (1994). The bifunctional iron-responsive element binding protein/cytosolic aconitase: The role of active-site residues in ligand binding and regulation. Proc. Natl. Acad. Sci, 91, 7321-7325. PDF
  
• Samaniego, F., Chin, J., Iwai, K., Rouault, T. A., and Klausner, R. D.
  (1994). Molecular Characterization of a Second Iron Responsive Element Binding Protein, Iron Regulatory Protein 2 (IRP2): Structure, Function and Post-translational Regulation. J. Biol. Chem. 269, 30904-30910.
  
• Klausner, R. D., and Rouault, T. A.
  (1993). A Double Life: Cytosolic aconitase as a regulatory RNA binding protein. Mol. Biol. of Cell 4, 1-5.
  Klausner, R. D., Rouault, T. A., and Harford, J. B.
  (1993). Regulating the fate of mRNA: the control of cellular iron metabolism. Cell 72, 19-28.
  
• Rouault, T. A., Tang, C. K., Kaptain, S., Burgess, W. H., Haile, D. J., Samaniego, F., McBride, O. W., Harford, J. B., and Klausner, R. D.
  (1990 Oct). Cloning of the cDNA encoding an RNA regulatory protein--the human iron-responsive element-binding protein. Proc Natl Acad Sci U S A 87, 7958-7962.
  
• Rouault, T. A., Hentze, M. W., Haile, D. J., Harford, J. B., and Klausner, R. D.
  (1989). The iron-responsive element binding protein: a method for the affinity purification of a regulatory RNA-binding protein. Proc. Natl. Acad. Sci. 86, 5768-5772.
  
• Rouault, T. A., Hentze, M. W., Caughman, S. W., Harford, J. B., and Klausner, R. D.
  (1988). Binding of a cytosolic protein to the iron-responsive element of human ferritin messenger RNA. Science 241, 1207-1210.

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